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- W2353624863 abstract "The construction of transfer vector,pBacPAK-His1-Sj23,is made through subcloning the Sj23 membrane protein gene from vector of pET28(c) into the transfer vector of pBacPAK-His1.The transfer vector pBacPaK-his1-Sj23 then co-trans-fected Bombyx mori cells with linealized virus of Bombyx mori nuclear polyhedrosis virus.The recombinant virus was got through PCR identification and the purified recombinant virus was got by blue and white plaque assay.SDS-PAGE shows that the (23 000) membrane protein was expressed in Bombyx mori cells,and the molecular weight is (26 000).The recombinant Sj23 can be recognized by the serum of rabbit suffered from the S.japonicum." @default.
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- W2353624863 date "2004-01-01" @default.
- W2353624863 modified "2023-09-26" @default.
- W2353624863 title "Expression and Identification of Gene Coding for Sj23 Antigen in Bombyx mori Cells" @default.
- W2353624863 hasPublicationYear "2004" @default.
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