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- W2353636791 abstract "To improve the efficiency of inducing mouse embryonic stem cell (ES cell) differentiation into cardiomyocytes,an efficiet system combining suspension culture with 0.8% DMSO induction was developed.Under this condition,rhythmic beating embryoid bodies were observed on day 9 and peaked on day 14 reaching 70% of rhythmic beating embryoid bodies.RT-PCR results revealed that mRNA level of cardiac muscle specific gene troponin I was increased as the times cultured with DMSO.Using Immunofluorescent technique,the expression of a cardiac specific marker α-actinin was found in the differentiated cells.At the same time clear sarconeres structure was visible in these differentiated cells under microscope.These results indicate that the ES cells could be efficiently induced into cardiomyocytes in this system." @default.
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- W2353636791 date "2005-01-01" @default.
- W2353636791 modified "2023-09-25" @default.
- W2353636791 title "Induced Differentiation of Mouse Embryonic Stem Cell into Cardiomyocytes" @default.
- W2353636791 hasPublicationYear "2005" @default.
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