Matches in SemOpenAlex for { <https://semopenalex.org/work/W2353663114> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W2353663114 abstract "AIM: To construct and express RNase-resistant virus-like particles containing the RNA fragments of SARS CoV. METHODS: The primers of MS2 assembly protein gene, coat protein gene and the fragment of SARS Cov RNA polymerase gene were synthesized according to their sequences from Genbank data and were used to amplify their cDNAs by RT-PCR. These cDNAs were purified with SABC's ResincolumTM system for DNA purification from agarose gel. Expression vector pTrc99a and their cDNA fragments were ligated together with T4 DNA ligase after digestion with restriction enzymes. The prokaryotic expression was obtained by the transformation of the recombinant plasmid constructed above into E.coli JM109. The obtained virus-like particles were purified and tested by quantitative analysis, RT-PCR and stable experiment. RESULTS: The RNase-resistant virus-like particles containing the RNA fragments of SARS CoV was successfully constructed and remained stable for 30 days at 37℃. CONCLUSION: The new-type virus-like particles can be conveniently used as positive control of SARS clinical nucleic diagnostic reagents." @default.
- W2353663114 created "2016-06-24" @default.
- W2353663114 creator A5010444377 @default.
- W2353663114 date "2004-01-01" @default.
- W2353663114 modified "2023-09-28" @default.
- W2353663114 title "Construction and expression of virus-like particles containing SARS CoV RNA" @default.
- W2353663114 hasPublicationYear "2004" @default.
- W2353663114 type Work @default.
- W2353663114 sameAs 2353663114 @default.
- W2353663114 citedByCount "0" @default.
- W2353663114 crossrefType "journal-article" @default.
- W2353663114 hasAuthorship W2353663114A5010444377 @default.
- W2353663114 hasConcept C104317684 @default.
- W2353663114 hasConcept C10879258 @default.
- W2353663114 hasConcept C128319531 @default.
- W2353663114 hasConcept C150194340 @default.
- W2353663114 hasConcept C153911025 @default.
- W2353663114 hasConcept C159047783 @default.
- W2353663114 hasConcept C187882448 @default.
- W2353663114 hasConcept C22744801 @default.
- W2353663114 hasConcept C24107716 @default.
- W2353663114 hasConcept C2522874641 @default.
- W2353663114 hasConcept C40767141 @default.
- W2353663114 hasConcept C552990157 @default.
- W2353663114 hasConcept C55493867 @default.
- W2353663114 hasConcept C67705224 @default.
- W2353663114 hasConcept C86803240 @default.
- W2353663114 hasConceptScore W2353663114C104317684 @default.
- W2353663114 hasConceptScore W2353663114C10879258 @default.
- W2353663114 hasConceptScore W2353663114C128319531 @default.
- W2353663114 hasConceptScore W2353663114C150194340 @default.
- W2353663114 hasConceptScore W2353663114C153911025 @default.
- W2353663114 hasConceptScore W2353663114C159047783 @default.
- W2353663114 hasConceptScore W2353663114C187882448 @default.
- W2353663114 hasConceptScore W2353663114C22744801 @default.
- W2353663114 hasConceptScore W2353663114C24107716 @default.
- W2353663114 hasConceptScore W2353663114C2522874641 @default.
- W2353663114 hasConceptScore W2353663114C40767141 @default.
- W2353663114 hasConceptScore W2353663114C552990157 @default.
- W2353663114 hasConceptScore W2353663114C55493867 @default.
- W2353663114 hasConceptScore W2353663114C67705224 @default.
- W2353663114 hasConceptScore W2353663114C86803240 @default.
- W2353663114 hasLocation W23536631141 @default.
- W2353663114 hasOpenAccess W2353663114 @default.
- W2353663114 hasPrimaryLocation W23536631141 @default.
- W2353663114 hasRelatedWork W1499797573 @default.
- W2353663114 hasRelatedWork W1964367017 @default.
- W2353663114 hasRelatedWork W1969900938 @default.
- W2353663114 hasRelatedWork W2012931451 @default.
- W2353663114 hasRelatedWork W2013544035 @default.
- W2353663114 hasRelatedWork W2035551526 @default.
- W2353663114 hasRelatedWork W2059864748 @default.
- W2353663114 hasRelatedWork W2061734037 @default.
- W2353663114 hasRelatedWork W2252543236 @default.
- W2353663114 hasRelatedWork W2351277928 @default.
- W2353663114 hasRelatedWork W2355794895 @default.
- W2353663114 hasRelatedWork W2357030831 @default.
- W2353663114 hasRelatedWork W2360082508 @default.
- W2353663114 hasRelatedWork W2376565523 @default.
- W2353663114 hasRelatedWork W2383610149 @default.
- W2353663114 hasRelatedWork W2388711659 @default.
- W2353663114 hasRelatedWork W2521989111 @default.
- W2353663114 hasRelatedWork W2710167666 @default.
- W2353663114 hasRelatedWork W2773526842 @default.
- W2353663114 hasRelatedWork W2820151400 @default.
- W2353663114 isParatext "false" @default.
- W2353663114 isRetracted "false" @default.
- W2353663114 magId "2353663114" @default.
- W2353663114 workType "article" @default.