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• W2353669458 abstract "ObjectivesTo identify successful transfection of FPRL1 gene into HEK293 cells by liposome method. MethodsG418 filtration, RT PCR and chemotaxis test were used.ResultsWith liposome method, FPRL1 gene was transfected into HEK293 cells;then G418 filtration was performed. The colony like cell clones were formed 3 wks later. RT PCR demonstrated that FPRL1 gene was transfected successfully and expressed in HEK293/FPRL1 cells. A band of object fragment with about 700 bp in size was visible on gel electrophoresis, whereas negative result was obtained in control group. Chemotaxis suggested that FPRL1 was expressed on the surface of HEK293/FPRL1 cells, which displayed chemotactic response to a certain concentration of fMLP; while HEK293 cells were non responsive.ConclusionThe results of RT PCR and chemotaxis test confirm that successful transfection of FPRL1 gene into HEK293 cells with its intracellular expression is achieved by liposome method." @default.
• W2353669458 created "2016-06-24" @default.
• W2353669458 creator A5090490027 @default.
• W2353669458 date "2002-01-01" @default.
• W2353669458 modified "2023-09-25" @default.
• W2353669458 title "Transfection of FPRL1 Gene into HEK293 Cells by Liposome Method and Its Identification" @default.
• W2353669458 hasPublicationYear "2002" @default.
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