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- W2353670087 abstract "Objective To clone,express and characterize the recombinant protein of the capsid protein VP1 of enterovirus 71(EV71),and lay the foundation for preparation of antibodies and diagnose reagents of EV71.Methods The VP1 gene was cloned into pET15b vector to construct the recombinant plasmid pET15b/VP1.Recombinant VP1 protein was expressed in E.coli BL21 and purified by metal(Ni2+) chelating affinity chromatography.The recombinant protein was determined by Western blot.ELISA was used to determine the antigenicity of the recombinant protein.Results The recombinant VP1 protein can be over expressed in E.coli.The molecular mass was estimated as 36 kDa by SDS-PAGE,and was consistent with the expected size.The antigenicity of the recombinant protein was demonstrated by ELISA.Conclusion The capsid protein VP1 of EV71 was successful cloned and expressed,which could be useful for developing diagnose reagents of EV71." @default.
- W2353670087 created "2016-06-24" @default.
- W2353670087 creator A5084477357 @default.
- W2353670087 date "2011-01-01" @default.
- W2353670087 modified "2023-09-27" @default.
- W2353670087 title "Expression,purification and characterization of the capsid protein VP1 of enterovirus 71 in E.coli" @default.
- W2353670087 hasPublicationYear "2011" @default.
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