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- W2353676594 abstract "Objective:To explore the formulation and storage methods for bacillus fastidious uricase as a diagnosis enzyme.Methods: Lyophilized powders of this uricase containing protective additives,and solutions of this uricase containing preservatives,were stored at-18℃ or 4℃.Enzyme activity was monitored at proper intervals.Homotetramers and homodimers of this uricase were resolved by polyacrylamide gel electrophoresis(PAGE),and were stained separately for both proteins and enzyme activities.Results:The lyophilized powders and solution preparations of this uricase yielded the corresponding residual enzyme activities of 12% and 100%,respectively,after storage at 4℃ for 28 days,and the residual enzyme activities of 24% and 92%,respectively,after storage at-18℃ for 28 days.The use of trehalose,sucrose and mannitol as the protective additives in lyophilized powders gave the residual enzyme activities of 100%,76% and 43%,respectively,after storage for 56 days at-18℃.The use of NaN3 and benzoic acid as the preservative in the solutions gave the residual enzyme activities of 99% and 82%,respectively,after storage for 56 days at 4℃.By PAGE analyses of lyophilized powders of low activity,less active homotetramers but more inactive homodimers were observed.Conclusion:This uricase as a diagnosis reagent can be prepared in solutions containing NaN3 for storage at 4℃." @default.
- W2353676594 created "2016-06-24" @default.
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- W2353676594 date "2012-01-01" @default.
- W2353676594 modified "2023-09-24" @default.
- W2353676594 title "Formulation and storage of bacillus fastidious uricase as diagnosis enzymes" @default.
- W2353676594 hasPublicationYear "2012" @default.
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