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- W2353722668 abstract "Objective To measure free Ca~(2+) concentration of cultured cardiac cells in neonatal rats ,and observe the effect of KCl,isoprenaline(Iso)in [Ca~(2+)]i , and approach initially their mechanism of action. Methods Rat neonatal cardiac myocytes were cultured, Fura-2/AM was loaded as calcium probe.The changes of cyosolic Ca~(2+) were observed by cation meaturement system. Results The value of Ca~(2+) was (83.3±11.67)nmol/L(±s) for 6 quiescent cardiac cells, KCl 30,50,and 70mmol/L triggered a sustained rise in [Ca~(2+)]i in concentration-dependent manner,the same isoprenaline 10mmol/L also produced a sustained elevation in [Ca~(2+)]i. Conclusions The system can accurately reflect cytosolic concentration of free Ca~(2+) ,KCl,Iso can increase [Ca~(2+)]i by different actiong mechanism." @default.
- W2353722668 created "2016-06-24" @default.
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- W2353722668 date "2004-01-01" @default.
- W2353722668 modified "2023-09-26" @default.
- W2353722668 title "The Effect of KCl and Iso on the Free Ca~(2+) Concentration of Single Cultured Cardiac Cell of the Neonate Rat" @default.
- W2353722668 hasPublicationYear "2004" @default.
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