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- W2353795634 abstract "To obtain non-pathogenic rabies virus glycoprotein(RV-G),we expressed RV-G in Saccaromyces Cerevisiae(S.cerevisiae).In our study,tat-G fusion gene was cloned into the expression vector pYes2.0,which allows expression of a foreign gene in the yeast cells under the control of GAl1 promoter.Transforma-tion was performed by using lithium-treated yeast cells and several Ura+-tranformants were isolated.Ac-cording to the relative mobility in SDS-PAGE,we know probably two forms(designated as yGI and yGⅡ) of RV-G analogues produced in S.cerevisiae,their molecular weights were estimated as 66 kD and 56 kD,respectively.On the other hand,there was a specific band about 56 kD shown in western blot result.Com-bining precursors’ achievements,we will draw a conclusion that trans-membrane domain(TD) and cyto-plasmic domain have a negative regulation on RV-G antigen immunogenicity in S.cerevisiae." @default.
- W2353795634 created "2016-06-24" @default.
- W2353795634 creator A5083918271 @default.
- W2353795634 date "2009-01-01" @default.
- W2353795634 modified "2023-09-27" @default.
- W2353795634 title "Expression of Rabies Virus Glycoprotein Gene in Saccharomyces cerevisiae" @default.
- W2353795634 hasPublicationYear "2009" @default.
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