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- W2353798477 abstract "Objective: To study the effects of 2 β_(1)-adrenergic receptor blockers(esmolol and landiolol) and 1 β_(2)-adrenergic receptor agonist(salbutamol) on the stimulus-induced superoxide anion generation in human neutrophils in vitro.Methods: Superoxide anion generation in human neutrophils stimulated by 3 β-adrenergic agents was determined by using cytochrome C reduction assay.The stimulation of human neutrophils with 3 β-adrenergic agents was induced by 3 stimuli,arachidonic acid(AA),N-formyl-methionyl-leucyl-phenylalanine(fMLP),and phorbol 12-myristate 13-acetate(PMA).The serine/threonine phosphorylation in neutrophils was detected by using Western blotting.Results: Esmolol inhibited the superoxide anion generation induced by AA in a concentration-dependent manner.Lnadiolol increased the superoxide anion generation induced by fMLP.Salbutamol inhibited the superoxide anion generation induced by fMLP and PMA in a concentration-dependent manner.The inhibition of AA-induced superoxide anion generation by esmolol [CM(5:46]was parallel to that of serine/threonine phosphorylation in neutrophils.Conclusion: The effect of β-adrenergic agents on stimulus-induced superoxide anion generation is independent on the types and blocking degree of β-adrenergic receptor." @default.
- W2353798477 created "2016-06-24" @default.
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- W2353798477 date "2006-01-01" @default.
- W2353798477 modified "2023-09-26" @default.
- W2353798477 title "Effect of β-adrenergic agents on the stimulus-induced superoxide anion generation in human neutrophils in vitro" @default.
- W2353798477 hasPublicationYear "2006" @default.
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