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- W2353805182 abstract "Viral vectors and plasmid vectors are the most frequently used gene expression vectors and they may cause unwanted side effects including immunologic,inflammatory reaction,cytotoxicity and gene silence.Here a new non-viral gene expression mini vector based on AAV-ITR is developed,and the ITRs will protect the mini vector from the degradation by nucleases in the cell for its T-shaped structure.The GFP is selected as reported gene for AAV-ITR mini vector.Single-stranded DNA is prepared by thermal denaturation method,and then the plasmid with GFP gene,double-stranded DNA vector and AAV-ITR mini vector are transfected intc eukaryotic cells respectively.The results were tested by fluorescence microscope and flow cytometry.The results show that the AAV-ITR mini vector has a higher efficiency of transfection and expression.It can be preliminarily concluded that AAV-ITR mini vector could be a new non-viral gene therapy vector with single strand." @default.
- W2353805182 created "2016-06-24" @default.
- W2353805182 creator A5004347888 @default.
- W2353805182 date "2013-01-01" @default.
- W2353805182 modified "2023-09-23" @default.
- W2353805182 title "Construction of a New Gene Delivering Mini Vector Based on AAV-ITR" @default.
- W2353805182 hasPublicationYear "2013" @default.
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