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- W2353807797 abstract "The proteins of photo-thermo-sensitive genetic mail sterile rice(ORYZA SATIVA,peiai64S) spikelet on fertility alternation stage were separated by two-dimensional electrophoresis with immobilized pH(3-10)gradients as the first dimension and SDS-PAGE as the second.2-D maps with high quality and reproducibility were obtained.Mean standard deviations of 1.49±0.22 mm for IPG-IEF and 1.24±0.18 mm for SDS-PAGE were obtained.60 protein spots out of about 1000 detectable spots on the 2-D gels were indentified by the following two methods:1)N-terminal Edman degradation microsequencing after the protein spots were electro-transferred to PVDF membrane.2)MALDI-TOF-MS peptide fingerprint analysis of the protein spots and protein database searching.The 2D protein maps of the rice spikelet during the sterile and fertile stages were compared.While the rice spikelet changed from sterile to fertile stages,about 16 protein spots showed variations that were significant and reproducible.Six up-regulated proteins and nine down-regulated proteins were identified.One protein only exist in fertile stage." @default.
- W2353807797 created "2016-06-24" @default.
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- W2353807797 date "2007-01-01" @default.
- W2353807797 modified "2023-09-25" @default.
- W2353807797 title "Preliminary Proteomic Analysis of the Protains of Photo-thermo-sensitive Genic Male Sterile RiceSpikelet on Fertility Alternation Stage" @default.
- W2353807797 hasPublicationYear "2007" @default.
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