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- W2353840902 abstract "AIM: To construct CTLA41g adenovirus vectors (AdCTLA4lg) by homologous recombination and study their activity, and to employ the vectors to induce cardiac transplantation tolerance by gene therapy. METHODS; CTLA4lg gene was cloned to pCA13 adenovirus shuttle plasmid by recombination strategy. Construction of CTLA4lg adenovirus vectors was performed by homologous recombination of pCA13 plasmid containing CTLA4lg gene with adenovirus helper plasmid, followed by packaged with 293 cells. Expression and secretion of CTLA4lg was confirmed by RT-PCR, SOS-PAGE and Western blot. The inhibitory effect of supernate of 293 cells infected with AdCTLA4lg on MLR in vitro was observed. A biological activity of CTLA4lg adenovirus vectors was determined by AdCTLA4lg gene therapy in rats in vivo. RESULTS; The Construction of CTLA4lg adenovirus vector was successful. It was confirmed that the supernatant of 293 cells infected with AdCTLA4lg could inhibit MLR in vitro. It was also showed that CTLA4lg adenovirus vectors could induce transplantation tolerance and prolong allograft survival when they were administrated in rats in vivo. CONCLUSION; CTLA4lg adenovirus vectors successfully constructed can infect 293 package cells and secrete CTLA4lg. The CTLA4lg protein can inhibit T cell activation. The CTLA4lg adenovirus vectors can be employed to gene therapy in vivo, and induce transplantation tolerance." @default.
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- W2353840902 date "2003-01-01" @default.
- W2353840902 modified "2023-09-28" @default.
- W2353840902 title "Construction and biological activities of CTLA4Ig adenovirus vectors" @default.
- W2353840902 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15132904" @default.
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