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- W2353886251 abstract "Sperm cells of rice ( Oryza sativa L.) were isolated from mature pollen grains near anthesis using osmotic shock with sucrose solution. They were separated from pollen contaminants by a Percoll gradient centrifugation. Isolated sperm cells were viable as tested with the fluorochromatic reaction. Cytological observations revealed that the two sperm cells just released from a pollen grain were linked together and each had a long tail_like extension. The isolated sperm cells were spherical with irregular surface and most of them separated, but some still remained in pairs that connected by finger_like structure. As observed with TEM, the isolated sperm cell was surrounded by a single plasma membrane and cell wall did not exist. Sperm nucleus was large and euchromatic." @default.
- W2353886251 created "2016-06-24" @default.
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- W2353886251 date "1999-01-01" @default.
- W2353886251 modified "2023-09-25" @default.
- W2353886251 title "Isolation and Cytological Observation of Viable Sperm Cells of Rice" @default.
- W2353886251 hasPublicationYear "1999" @default.
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