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- W2353935404 abstract "Factors affecting oocytes in vitro maturation(IVM)from different size of follicles and cryopreservation of oocytes in different development phases were studied.The results showed that the maturation rate of IVM was very significantly higher in the medium containing 10%ECS(72 86%)than that containing 10%NCS(62 21%)(P0 05).Addition of 10% porcine follicular fluid(pFF)to mature medium inhibited the in vitro maturation of oocytes.Oocytes from different size of follicles had different development ability.Oocytes collected from big(5 mm)and medium(2?5 mm)follicles had a higher maturation rate than oocytes from small(2 mm)follicle (P0 05).Oocytes in MⅡ stage had higher antifreezing ability than GV stage and oocytes culture for 24 h.The survival rate of oocytes after cryopreservation and thawing was 35 59%,significant higher than oocytes cryopreservation in GV stages(24 64%)and culture for 24 h(23 36%)(P0 05).The rate of morphological normality after thawing was significantly lower in group of GV stages(53 24%)than in group of MⅡ stage(72 81%)and culture for 24 h(77 22%)(P0 05)." @default.
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- W2353935404 date "2002-01-01" @default.
- W2353935404 modified "2023-09-25" @default.
- W2353935404 title "Primary study on in vitro maturation and cryopreservation of the porcine follicular oocytes" @default.
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