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- W2353960571 abstract "Based on the NcSRS2 gene sequence of Neospora caninum in the Genbank,a pair of primers containing EcoRⅠand NotⅠenyme digestion sites were designed.Using the Neospora caninum DNA,the ORF region of NcRSR2 gene was amplified by polymerase chain rection(PCR)and then inserted into PMD18-T Simple vector.Then the plasmid DNA was digested with Eco RⅠ and NotⅠ,and the prokaryotic expression vector PGEM-4T-2 was prepared,the gene was cloned into the vector.Finally a recombinant plasmid named PGEX-NcRSR2 was obtained,and was identified by PCR,restriction enzyme analysis and sequencing.The accuracy of recombinant plasmid was confirmed." @default.
- W2353960571 created "2016-06-24" @default.
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- W2353960571 date "2008-01-01" @default.
- W2353960571 modified "2023-09-28" @default.
- W2353960571 title "Construction of Prokaryotic Expression Plasmid of NcSRS2 Gene of Neospora caninum" @default.
- W2353960571 hasPublicationYear "2008" @default.
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