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- W2354012010 abstract "Objective To study characteristics of immunolabling of cochlear ribbon synapses in adult C57BL/6J mice and its significance in research.Methods Fresh apical coil organ of Corti specimens were obtained for whole mount staining with Ctbp2 or GluR2/3 alone,or in various combinations including Ctbp2 with Dapi,GluR2/3 with Dapi,Ctbp2 with GluR2/3 and Ctbp2 with GluR2/3 plus Dapi.Cochlear ribbon synapses were examined using a confocal microscope for patterns of immunostaining.Results Ctbp2 stainging highlighted spots where cochlear ribbon synapses were located and anatomical relations were shown by Ctbp2 and Dapi double staining.When Ctbp2 was combined with GluR2/3,the entire cochlear ribbon synapse was demonstrated,with improved illustration of anatomical connections between ribbon synapses and hair cells in the cochlea when Dapi was added.Conclusion This study demonstrates a useful method for studying ribbon synapse of cochlear inner hair cells,which may benefit future studies." @default.
- W2354012010 created "2016-06-24" @default.
- W2354012010 creator A5011681363 @default.
- W2354012010 date "2010-01-01" @default.
- W2354012010 modified "2023-09-27" @default.
- W2354012010 title "Immunostaining of cochlear ribbon synapse in adult C57BL/6J mice" @default.
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