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- W2354030551 abstract "[Objective]To observe the humoral or cellular immune responses,the HCMV IE1 DNA vaccine is injected by intramuscular cells in BALB/c mice,and it is expected to provide experimental basis for further studying DNA vaccine against HCMV infection.[Methods]BABL/c mice were immunized with pcDNA3.1(-)-IE1 or pcDNA3.1(-) or PBS buffer by intramuscular cells at 2-week interval for four times.Two weeks after the last time of immunization,HCMV IE1 gene or protein in intramuscular cells was detected by PCR or observed with immunohistochemistry.Two weeks after the last time of immunization,ELISA was used to detect the quantities of IL-4,IL-2 or IFN-γ in the supernatant of murine spleen lymphocyte culture after stimulated by PHA.The proliferation response of spleen cells was detected using MTT assay,and the stimulation indexes(SI) were counted.All of data were analyzed by SPSS13.0.[Results]In the intramuscular cells of immunized mice,the fragments were consistent to HCMV IE1 gene detected by PCR.The result of immunohistochemistry showed that HCMV IE1 gene could express in intramuscular cells of mice.CKs in mice inoculated with pcDNA3.1(-)-IE1 were much more than those with pcDNA3.1(-) or with PBS,it was significant difference between the experiment group and control group(IL-4:P﹤0.05,IL-2 and IFN-γ:P﹤0.01);The stimulation index(SI) of spleen cells of DNA vaccine group was significantly higher than those of pcDNA3.1(-) group or PBS group(P﹤0.01) .[Conclusion]HCMV IE1 gene could exist constantly and express in intramuscular cells of BALB/c mice.The level of IL-4,IL-2 or IFN-r and stimulation indexs of spleen cells could be increased in BALB/c mice immunized with pcDNA3.1(-)-IE1." @default.
- W2354030551 created "2016-06-24" @default.
- W2354030551 creator A5029509600 @default.
- W2354030551 date "2011-01-01" @default.
- W2354030551 modified "2023-09-22" @default.
- W2354030551 title "IMMUNOCOMPETENCE STUDY OF HUMAN CYTOMEGALOVIRUS IE1 DNA VACCIN IN MICE" @default.
- W2354030551 hasPublicationYear "2011" @default.
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