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- W2354037642 abstract "Objective To explore a suitable method to detect predendritic cell(pDC) subsets in cord blood,peripheral blood,bone marrow and G-CSF-mobilized blood.Methods pDC subsets in cord blood,peripheral blood,bone marrow and G-CSF-mobilized blood were detected gated by Lin~-HLA-DR~+ cell population or CD34~-Lin~-HLA-DR~+ cell population.pDC1/pDC2,CD34~-Lin~-HLA-DR~+ cell/mononuclear cell(MNC) and Lin~-HLADR~+ cell/MNC,pDC/CD34~-Lin~-HLA-DR~+ cell and pDC/Lin~-HLA-DR~+ cell were compared between two gating methods.Results pDC1/pDC2 ratio in cord blood,peripheral blood,bone marrow and G-CSF-mobilized blood, CD34~-Lin~-HLA-DR~+ cell/MNC ratio and Lin~-HLA-DR~+ cell/MNC ratio,pDC/CD34~-Lin~-HLA-DR~+ cell ratio and pDC/Lin~-HLA-DR cell ratio in peripheral blood were not remarkably distinct between two gating methods(P0.05),but detecting CD34~-Lin~-HLA-DR~+ cell/MNC were lower than Lin~-HLA-DR~+ cell/MNC(P0.05),pDC/CD34~-Lina-HLA-DR~+ cell were higher than pDC/Lin~-HLA-DR~+ cell in cord blood,bone marrow and G-CSF-mobilized blood(P0.05).Conclusion pDC subsets in cord blood,bone marrow and G-CSF mobilized blood should be analyzed by gating by CD34~-Lin~-HLA-DR~+ cell population,and pDC subsets in peripheral blood can be analyzed by gating by Lin~-HLA-DR~+ cell population or CD34~-Lin~-HLA-DR~+ cell population because enriching CD34~+ cells gated by Lin~-HLA-DR~+ cell population were very fewer than in cord blood,bone marrow and G-CSF-mobilized blood." @default.
- W2354037642 created "2016-06-24" @default.
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- W2354037642 date "2007-01-01" @default.
- W2354037642 modified "2023-09-25" @default.
- W2354037642 title "Analysis of predendritic cell subsets in cord blood,peripheral blood and bone marrow" @default.
- W2354037642 hasPublicationYear "2007" @default.
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