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- W2354044189 abstract "Objective:To study the effect of low frequency vibration on cultured human osteoblasts in proliferation and differentiation. Methods: Osteoblasts isolated from the iliac trabecular bone specimens were treated with collagenase-trypsin, were then purified and cultured,and subsequently,osteoblasts were dealt with under low frequency vibration(0.1,0.2,0.5,2 and 5Hz)for 5d. The flow cytomety were used to observe the effect on the proliferation of osteoblasts. The biochemical indexes - alkaline phosphatase(ALP) and osteocalcin were assayed by spectrohotometric methods and radioimmunoassay respectively. Results :0.2 and 0.5Hz vibration could markedly augment osteoblast ALP activity(P0.05) ;0.1 and 2Hz vibration had no obvious effects on osteoblast ALP activity(P0. 05);5Hz distinctly reduced ALP activity (P0.05).0.2 and 0. 5Hz vibration could significantly increase cellnumbers of S phase from 10.4% to 12.45% and 16.12% , PI value from 20.14% to 26.21% and 28.75% respectively; 0. 1 and 2Hz vibration had no obvious effects on osteoblast proliferation index; 5Hz obviously decreased PI value to 13.22%(P0.01). 0.2 and 0.5Hz vibration could increase OCN release to 1.87 and 2.47ng/ml while, 2Hz and 5Hz all decrease it markedly(P 0.05). Conclusion: 0.2 - 0. 5Hz vibration could accelerate the osteoblast proliferation and differentiation, as well as ALP activity and OCN release." @default.
- W2354044189 created "2016-06-24" @default.
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- W2354044189 date "2003-01-01" @default.
- W2354044189 modified "2023-09-25" @default.
- W2354044189 title "Effects of Low Frequency Vibration on Human Osteoblasts" @default.
- W2354044189 hasPublicationYear "2003" @default.
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