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- W2354052007 abstract "[Objective] To explore the differentiation potency of skin analog reconstructed with ES cell-derived epidermal stem cells and dermal analog in hypodermis. [Methods] E14-ES cells, labeled with Hoechst 33342, were cocultured with human amnion. Four days later, epidermal stem cell clones were formed. The skin analog was reconstructed with ES-derived epidermal stem cells and dermal analog, which reconstructed with 129 mice fetus fibroblasts and compound gel-gelatin sponge. The skin analogs were transplanted into 129 mice hypodermis. The differentiation tissues of skin analog were sampled at 2w, 4w, 6w, and 8w. Sections were observed with HE staining, immunohistochemical and di-labeled immunofluorescence methods to test the expression of β1 integrin, CK15, CK19, CEA, and CK18. [Results] Sections showed tubular or follicular like structure formed with simple or stratified epithelium at 2 and 4 weeks. Keratinized stratified squamous epithelium, sweat gland-like, sebaceous gland-like and hair follicle-like structures were observed at 6 week and 8 week after transplantation. Cells labeled by Hoechst 33342, formed tubular or follicular-like structure, expressed β1 integrin, CK15, CK19, CEA and CK18 positive respectively at 2w and 4w. The sweat glands-like structure expressed CEA and CK18 positive respectively at 6w and 8w. [Conclusion] Skin analogs reconstructed with ES-derived epidermal stem cells and dermal analogs can differentiate into keratinized stratified squamous epithelium, sweat gland-like, sebaceous gland-like and hair follicle-like structures in hypodermis." @default.
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- W2354052007 date "2007-01-01" @default.
- W2354052007 modified "2023-09-28" @default.
- W2354052007 title "Differentiation of Skin Analogs Reconstructed with ES Cell-Derived Epidermal Stem Cells and Dermal Analog" @default.
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