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- W2354060816 abstract "Two monoclonal antibodies (McAbs) against Aoc chain's C terminus offibrinogen (Fg) have been prepared and designated SZ-78 and SZ-79. Both the antigens in binding assay and immunoblot analysis showed that the two McAbs recognized the epitopes located in residues 549-560 of the A a chain. The two McAbs could accelerate rate of fibrin polymer assembly both in the purified system and in the human plasma. From the pictures of transmission electronmicioscopc, the average diameters of the fibers increase significantly to an average diameters of 375 nm after incubation with the McAbs, while it was only 75nm without addition of the McAbs. There were also more branchings of fibers with addition of McAbs. These observations demonstrate that the amino acid sequences of a 549-560 in the COOH terminus of the Aa chain may play an important role in the assembly of a fibrin clot, presumably being involved in lateral aggregation of protofibrils. The preparation of the McAbs supplies a usuful probe for the investigation of the functions of Aa chain of Fg." @default.
- W2354060816 created "2016-06-24" @default.
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- W2354060816 date "1995-01-01" @default.
- W2354060816 modified "2023-09-22" @default.
- W2354060816 title "PREPARATION AND CHARACTERIZATION OF TWO MONOCLONAL ANTIBODIES AGAINST A_α CHAIN'S C TERMINUS OF FIBRINOGEN" @default.
- W2354060816 hasPublicationYear "1995" @default.
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