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- W2354071743 abstract "ObjectiveTo study the differentiation of adipose tissue-derived mesenchymal stem cells (ADMSCs) into cardiomyocytes in vitro. MethodsADMSCs were isolated and purified by the method of digesting and adhering to the culture plastis. The third generation of cells were determined with immunocytochemistry method and induced by 5-aza. On the 7th, 14th, 21st, 28th day after being induced, the surface antigen of myoblast cell and cardiac myocyte were determined, the expression of gene of GATA4 and Nkx2.5 were tested, and the content of atrial natriuretic polypeptide (ANP) assayed. ResultsImmunocytochemical staining showed CD_ 44, CD_ 13, CD_ 105 positive, CD_ 45, CD_ 34, HLA-DR, factor Ⅷ negative. After being induced by 5-aza, the direction of cell arraying was gradually similar. The cells were stained positively for Desmin, α-Sarcomeric Actin, myosin heavy chain and Troponin T. The cells express GATA4 and Nkx2.5 and secrete ANP. ConclusionThere was mesenchymal stem cells in human adipose tissue. 5-aza may induce ADMSCs to differentiate into cardiomyocytes in vitro." @default.
- W2354071743 created "2016-06-24" @default.
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- W2354071743 date "2006-01-01" @default.
- W2354071743 modified "2023-09-24" @default.
- W2354071743 title "Inducing Mesenchymal Stem Cells from Adult Human Adipose Tissue to Differentiate into Cardiomyocytes In Vitro" @default.
- W2354071743 hasPublicationYear "2006" @default.
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