Matches in SemOpenAlex for { <https://semopenalex.org/work/W2354095715> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2354095715 abstract "Objective To explore the effect of Astragalus Membranaceus(AM)on rats'hepatic oval cell growth cultured in vitro.Methods The rats were fed with 0.1% ethonine contained choline deficient diet for 4—6 weeks.Hepatic oval cells were isolated from rats'liver.Reverse transcription-polymerase chain reaction(RT- PCR),immunohistology,Western blot method were used to determine the cultured cells.Effect of AM in different concentrations on hepatic oval cell growth was observed by MTT method.Changes of cell phenotype and cell cycle were respectively observed by Giemsa staining and fluorescence activated cell sorter(FACS).Results The isola- ted cells not only expressed stem cell marker c-kit,but also expressed embryonic hepatic cell markerα-fetoprotein, cholangiocyte marker OV6 and CK19,as well as mature hepatic cell marker albumin,which were thought to be rats' hepatic oval cells.AM from the concentration of 0.125 mg/ml to 4 mg/ml had the stimulating effect on the growth of hepatic oval cells at 48 hrs,among which AM of 2 mg/ml had the most obvious stimulating growth effect (P＜0.05).Some proliferation enhanced cells appeared treated by AM of 2 mg/ml 48 hrs later.So did some cells of lower nucleoplasmie ratio.The cell cycle determination showed that cells treated with AM in G2-S and M phase were increased more than the control cells without treatment by AM.Conclusion AM has obvious stimulating effect on the growth of hepatic oval cells cultured in vitro." @default.
- W2354095715 created "2016-06-24" @default.
- W2354095715 creator A5006163152 @default.
- W2354095715 date "2005-01-01" @default.
- W2354095715 modified "2023-09-26" @default.
- W2354095715 title "The Stimulation of Astragalus Membranaceus on Rats' Hepatic Oval Cell Growth Cultured in vitro" @default.
- W2354095715 hasPublicationYear "2005" @default.
- W2354095715 type Work @default.
- W2354095715 sameAs 2354095715 @default.
- W2354095715 citedByCount "0" @default.
- W2354095715 crossrefType "journal-article" @default.
- W2354095715 hasAuthorship W2354095715A5006163152 @default.
- W2354095715 hasConcept C104317684 @default.
- W2354095715 hasConcept C126322002 @default.
- W2354095715 hasConcept C134018914 @default.
- W2354095715 hasConcept C142724271 @default.
- W2354095715 hasConcept C1491633281 @default.
- W2354095715 hasConcept C153911025 @default.
- W2354095715 hasConcept C16685009 @default.
- W2354095715 hasConcept C176891718 @default.
- W2354095715 hasConcept C188947578 @default.
- W2354095715 hasConcept C202751555 @default.
- W2354095715 hasConcept C204787440 @default.
- W2354095715 hasConcept C2583947 @default.
- W2354095715 hasConcept C2776415932 @default.
- W2354095715 hasConcept C2780798337 @default.
- W2354095715 hasConcept C29537977 @default.
- W2354095715 hasConcept C55493867 @default.
- W2354095715 hasConcept C62112901 @default.
- W2354095715 hasConcept C71924100 @default.
- W2354095715 hasConcept C86803240 @default.
- W2354095715 hasConceptScore W2354095715C104317684 @default.
- W2354095715 hasConceptScore W2354095715C126322002 @default.
- W2354095715 hasConceptScore W2354095715C134018914 @default.
- W2354095715 hasConceptScore W2354095715C142724271 @default.
- W2354095715 hasConceptScore W2354095715C1491633281 @default.
- W2354095715 hasConceptScore W2354095715C153911025 @default.
- W2354095715 hasConceptScore W2354095715C16685009 @default.
- W2354095715 hasConceptScore W2354095715C176891718 @default.
- W2354095715 hasConceptScore W2354095715C188947578 @default.
- W2354095715 hasConceptScore W2354095715C202751555 @default.
- W2354095715 hasConceptScore W2354095715C204787440 @default.
- W2354095715 hasConceptScore W2354095715C2583947 @default.
- W2354095715 hasConceptScore W2354095715C2776415932 @default.
- W2354095715 hasConceptScore W2354095715C2780798337 @default.
- W2354095715 hasConceptScore W2354095715C29537977 @default.
- W2354095715 hasConceptScore W2354095715C55493867 @default.
- W2354095715 hasConceptScore W2354095715C62112901 @default.
- W2354095715 hasConceptScore W2354095715C71924100 @default.
- W2354095715 hasConceptScore W2354095715C86803240 @default.
- W2354095715 hasLocation W23540957151 @default.
- W2354095715 hasOpenAccess W2354095715 @default.
- W2354095715 hasPrimaryLocation W23540957151 @default.
- W2354095715 hasRelatedWork W1981154737 @default.
- W2354095715 hasRelatedWork W2032642132 @default.
- W2354095715 hasRelatedWork W225613825 @default.
- W2354095715 hasRelatedWork W2349130519 @default.
- W2354095715 hasRelatedWork W2350350605 @default.
- W2354095715 hasRelatedWork W2351445332 @default.
- W2354095715 hasRelatedWork W2352222583 @default.
- W2354095715 hasRelatedWork W2353737016 @default.
- W2354095715 hasRelatedWork W2354728441 @default.
- W2354095715 hasRelatedWork W2357546538 @default.
- W2354095715 hasRelatedWork W2364542117 @default.
- W2354095715 hasRelatedWork W2374973244 @default.
- W2354095715 hasRelatedWork W2375256727 @default.
- W2354095715 hasRelatedWork W2376158490 @default.
- W2354095715 hasRelatedWork W2381884089 @default.
- W2354095715 hasRelatedWork W2384170072 @default.
- W2354095715 hasRelatedWork W2390201402 @default.
- W2354095715 hasRelatedWork W2390432803 @default.
- W2354095715 hasRelatedWork W2443287364 @default.
- W2354095715 hasRelatedWork W169406307 @default.
- W2354095715 isParatext "false" @default.
- W2354095715 isRetracted "false" @default.
- W2354095715 magId "2354095715" @default.
- W2354095715 workType "article" @default.