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- W2354114618 abstract "Transgenic plants at a frequency of about 2.5% in an important crop, oilseed rape (Brassica napus), have been obtained through Agrobacterium-mediated transformation of cotyledon-petiole explants using two cosmid-type binary vectors (pOCA18 and pOCA28) that carry a kanamycin resistant gene (NPTII). It was observed that two Agrobocterium strains, an agropine-type strain (EHA105) and a nopaline-type strain (GV3101) and two cosmid vectors (pOCA18 and pOCA28) can be used to obtain transgenic plants. The integration of T-DNA in the B. napus genome was confirmed by Southern blot analysis, and the data suggested that there were one to four copies of T-DNA per integrated T-DNA locus." @default.
- W2354114618 created "2016-06-24" @default.
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- W2354114618 date "1996-01-01" @default.
- W2354114618 modified "2023-09-23" @default.
- W2354114618 title "Agrobacterium -mediated transformation of oilseed rape (Brassica napus) with cosmid-type binary vectors" @default.
- W2354114618 hasPublicationYear "1996" @default.
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