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- W2354162945 abstract "Objective:To investigate modulation of a specific HDAC inhibitor,Valproate acid sodium(VPA),on expression of Caspase3,Caspase8,Caspase9 by inhiting HDAC,as well as apoptosis rate of cancer cells treated with VPA and the specific inhibitors of Caspase3,Caspase8,Caspase9.Methods:Heptocellular carcinoma cells-HepG2,gastric carcinoma cells-BGC-823 and breast cancer cells-MCF-7 were cultured with 0.75-4.0 mmol/L of VPA for 48 hrs in vivo,apoptosis was analyzed by flow cytometry with Annexin V/PI assay.The activities and protein expressions of Caspase3,Caspase8,Caspase9 were detected by spectrophotometry and indirect immunofluorescence technique.Results:Contrary to control groups,VPA at concentrations between 0.75 and 4.0 mmol/L induced a significant apoptosis in HepG2,BGC-823,MCF-7 cells(P0.001) and the effect was dose-dependent.The activities and protein expressions of Caspase3,Caspase9 were up-regulated in HepG2,BGC-823,MCF-7 cells(P0.001);Capase8 was up-regulated at protein level in BGC-823 cells lightly,but it did not show any difference in HepG2 and MCF-7 cells(P0.05).The apoptosis rates of cancer cells treated with VPA and specific inhibitors of Caspase3,Caspase9 together was lower than in the groups with VPA treatment singlely(P0.005),thus indicationg that the activities of Caspase3,Caspase9 which had been activated by VPA were blocked by the specific inhibitors of Caspase3 and Caspase9.The apoptosis rate of the cells treated with VPA and Caspase8 specific inhibitor remained the same as the treated by VPA only,hence it was verified that Caspase8 activation did not play an important role in apoptosis induction by VPA in HepG2,BGC-823,MCF-7 cells.Conclusion:Modification of histone acetylizad level with VPA can regulate Caspase3,Caspase9 expressions obviously;To Caspase8,it show some difference according to the histogenesis and phenotypes of different carcinoma types.Up-regulation of the activities and protein expressions of Caspase3,Caspase9 may be the main pathway in apoptosis induction by VPA." @default.
- W2354162945 created "2016-06-24" @default.
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- W2354162945 date "2009-01-01" @default.
- W2354162945 modified "2023-09-25" @default.
- W2354162945 title "Modulation of Caspase expression by up-regulating histone acetylizad level with VPA" @default.
- W2354162945 hasPublicationYear "2009" @default.
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