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- W2354216047 abstract "Optimal conditions for protoplast formation and regeneration of Streptomyces cattleya were investigated. YEME medium supplemented with 0.2%(w/v) of glycine and 35%(w/v) of sucrose was found to be more efficient for protoplast formation that the medium S which has conventionally been used for protoplast formation of other Streptomyces species. Porotoplast formation and regeneration could most efficiently by carried out with the mycelia harvested at late exponential and the transitional period to stationary growth phase, respectively. For efficientregeneration, a hypertonic medium(R1) containing high levels of sucrose concentration was required due to a high reversion rate of the cells. Regeneration efficiency was observed to increase upto 500 fold at 0.6-0.8M of sucrose concentration, compared to 0.3M inthe original okanish's medium. Other conditions such as metal ion requirement, temperature effect on the regeneration were also studied." @default.
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- W2354216047 date "1987-01-01" @default.
- W2354216047 modified "2023-09-24" @default.
- W2354216047 title "A Study on Optimal Conditions for Protoplast Formation and Regeneration of Streptomyces cattleya" @default.
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