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- W2354220902 abstract "Objective To study whether the genes of HPI island of Yersinia is existed in enteric Shigelloid toxin producing and invasive Escherichia coli (ESIEC). Methods PCR amplification and Southern blots. Results The PCR results showed that 35% ESIEC strains ( n =43) yielded irp1, irp2 and fyuA fragments, of which the molecular size were identical to those of Yersinia enterocolitica WA. All of the E.coli strains tested were negative for other genes of HPI island which were existed in Y. pestis but not in Y.enterocolitica . The two E.coli strains with irp1, irp2 and fyuA gene were selected for further study. The chromosomal DNA was digested by EcoR Ⅰand hybridized with irp1, irp2 and fyuA DNA probes respectively, which were synthesized by PCR with chromosomal DNA of Y. enterocolitica as template. When fyuA probe was used, the Southern hybridization patterns of the two E. coli strains were identical to that of Y. enterocolitica WA. When irp1 and irp2 probe were used, the molecular size of hybridized fragments were different from that of Y. enterocolitica WA. Conclusion The HPI island of Y. enterocolitica was found to exist in 35% of ESIEC strains tested, and variation in restriction sites of various genes was observed." @default.
- W2354220902 created "2016-06-24" @default.
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- W2354220902 date "2000-01-01" @default.
- W2354220902 modified "2023-09-26" @default.
- W2354220902 title "The high-pathogenicity island of Yersinia enterocolitica species existed in enteric Shiga-like-toxin producing and invasive Escherichia coli" @default.
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