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- W2354221519 abstract "Polyunsaturated fatty acids(PUFAs) have remained as a natural product due to the synthetic difficulty of reproducing the methylene interrupted double bond sequence by industrial chemistry.Their significance to animals and humans lies in their biological activity,as precursors for groups of nutritionally important compounds and as essential cellular components.Interest in the production of PUFAs,especially DHA and EPA,from other sources for use in aquaculture feeds and human health supplements has fulled recent researches into the molecular biology of PUFA production in sea ice microorganisms.In Antarctic,the geography and climate differ from those in other places,and the bacteria there have adapted well to the environment there.Two hundred strains of bacteria were isolated from the sea ice in Antarctica(68°30′E,65°00′S) during the 19th Antarctic Scientific Expedition of China(2001-2002).The bacteria were screened for DHA by means of GC,with fish oil as the standard.Seven strains containing DHA or EPA were obtained,among which the strain of No.N-6 was outstanding.And its component of DHA was identified by GC-MS.According to the analysis,the relative content of DHA in N-6 was 8.72%,and total lipids in dry bacteria was 22.54%.The effects of some factors,including salinity,temperature and pH value,on the growth and DHA-content of strain N-6 were studied.The results show that the DHA-content is relatively high when in high salinity,low temperature and high pH,and the bacterium is psychrophilic,alkalophilic and halophilic.As fish oil fails to meet the increasing demand for purified DHA,alternative sources are being sought,and some microalgae containing DHA were screened.But there are some limitations in cultivation on a large scale,including sunlight,growth rate,etc.,all of which can be easily solved with the psychrophilic N-6 if cultured in chemostat at a low temperature,and the advantage of running the bioreaction at low temperature is avoiding the risk of contamination by other microorganisms.The purpose of this study on screening DHA-producing Antarctic bacteria is very cheerful,and much more of their talents will be exploited in future.[Journal of Fishery Sciences of China,2006,13(1):79-84]" @default.
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- W2354221519 date "2006-01-01" @default.
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- W2354221519 title "Screening on DHA-producing Antarctic bacteria and its cultural conditions" @default.
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