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- W2354256914 abstract "Objective:To analysis the SGPs(Salivary Gland Progenitor cells)isolated from injured SpragueDawlye(SD)rat submandibular gland.Methods:Tissue damage was induced by ligation of main duct from SD rats.Cells isolated with mechanical method and enzyme digestion were cultured.With limited dilution,cell lines purified from epithelium-like colonies were designated as rat SGPs and their characteristics were analyzed by antibodies of α6β1integrin,LN,CD29,CK19with immunofluorescent stainings.CD29,CD90.1andα6β1integrin were analysed with flow cytometry.Results:The SGPs expressedα6β1integrin,LN,CD29and CK 19.Flow cytometric analysis demonstrated that the percentage of positive cells forα6β1integrin,CD29,CD90.1,and D29/CD90.1combination were 94.99±4.04%,97.41±3.15%,98.81±1.58%,and 97.15±3.43%,respectively.Conclusion:The SGPs isolated from the SD rat salivary gland had characteristics of tissue stem cells." @default.
- W2354256914 created "2016-06-24" @default.
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- W2354256914 date "2010-01-01" @default.
- W2354256914 modified "2023-09-26" @default.
- W2354256914 title "Flow Cytometric and Immunohistochemistry analysis of Salivary Gland Progenitor Cells Induced by Tissue Damage" @default.
- W2354256914 hasPublicationYear "2010" @default.
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