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- W2354257684 abstract "【Objective】The objective of the experiment is to screen,sequence and bioinformatical analyze the ACCase clone from a BAC library of the dairy cow rumen microbiota.【Method】ACCase gene was screened from BAC library of the rumen microbiota by PCR sequence-driven analysis.Whole insert sequence of screened clone was sequenced by shotgun method.Then it was analyzed by gene annotation,blast and phylogenetic tree analysis.【Result】One ACCase clone(U12)was obtained,and its insert fragment (URE12)had the length of 43 358 bp and GC content of 43.75%.URE12 contained 38 CDSs predicted by GeneMark software,and it was probably originated from Proteobacteria by Signature analysis.ACCase encoded by Acc-32 was predicted to be 159 amino acids with a molecular weight of 17.89 kD and pI of 5.27.Acc-32 had the similarity of 41%with that from Elusimicrobium minutum.The combining site AA residue of Acc-32 was GQVICVIEAMKVFNELKA,and Acc-32 belonged toe-Proteobacteria by phylogenetic tree analysis.【Conclusion】The fragment containing ACCase was screened,and ACCase had some novel gene characteristics." @default.
- W2354257684 created "2016-06-24" @default.
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- W2354257684 date "2011-01-01" @default.
- W2354257684 modified "2023-10-03" @default.
- W2354257684 title "Screening and Bioinformatics Analysis of ACCase from a BAC Library of Dairy Cow Rumen Microbiota" @default.
- W2354257684 hasPublicationYear "2011" @default.
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