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- W2354305393 abstract "Objective To investigate the hepatocareinoma therapy by RNA interference,we used siRNA to inhibit ICAM-1 gene expression in vitro.Methods Plasmids pGenesil-1/ ICAM-1carried shRNA for ICAM-1 gene were transfected to HepG2 cells by using Lipofectamine TM2000.The mortality rate of HepG2 cells was determined by MTT staining,the protein level of ICAM-1 in the culture supernatant was measured with a enzyme-linked immunosorbent assay(ELISA).Results The recombinant plasmid pGenesil-1/ ICAM-1was constructed successfully.ELISA showed that pGenesil-1/ ICAM-1 vector mediated ICAM-1 siRNA could inhibit the expression of ICAM-1 in HepG2 cells.Conclusion siRNA targeting ICAM-1 mRNA can specially suppress the expression of targeting gene in HepG2 cells and cell growth." @default.
- W2354305393 created "2016-06-24" @default.
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- W2354305393 date "2006-01-01" @default.
- W2354305393 modified "2023-09-26" @default.
- W2354305393 title "Inhibition of ICAM-1 gene expression in liver cancer cells by shRNA" @default.
- W2354305393 hasPublicationYear "2006" @default.
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