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- W2354306255 abstract "Objective:To explore the activation of CD8+T cells of hepatocellular carcinoma(HCC) stimulated by the artificial antigen presenting cells(aAPC)(K32/4-IBBL/CD86).Methods: Peripheral CD8+T cells of HLA-A*0201 positive HCC patients were enriched by MACS separation system.CD8+T cells were cultured with aAPC at different ratios(3∶1,2∶1 and 1∶1),and CTLs were induced.Growth curve of CTLs was examined by trypan blue exclusion assay;MTS/PMS assay was used to detect the proliferation of CTLs;the IFN-γ secreting CTLs were assayed by FACS;the cytotoxicity of CTLs on a human liver cancer cell line BEL7402 and self-HCC cells was tested using MTT method.Results: The proliferation of peripheral CD8+ T cells stimulated with aAPC was significantly increased.At days 8,the CTL cell numbers of aAPC to CD8+ T cells at 1∶1,1∶2,1∶3 groups was significantly higher than those in the control group([21.2±2.5]×105,[17.6±3.2]×105,[15.3±2.8]×105 vs [8.5±0.15]×105,P0.01);and the proportion of IFN-γ secreting CTLs in 1∶1,1∶2,1∶3 groups was higher than those in the control group([26.2±1.91]%,[21.3±1.38]%,[18.6±1.20]% vs [0.1±0.02]%,P0.01).The cytotoxicity of aAPC-activated CTLs against BEL7402 cells and self-HCC cells in 1∶3 group was stronger than that in the control group([21.8±4.3]% vs [3.8±1.8]%,P0.01;[25.6±3.6]% vs [3.8±2.0]%,P0.01).A higher cytotoxic rate on BEL7402 cells and self-HCC cells in the 1∶1 group at a ratio of E(effect): T(target) as 50∶1 was achieved compared with that in the 1∶2 and 1∶3 groups([56.8±3.0]% vs [44.3±2.6]%,[38.9±4.7]%,P0.05;[64.8±4.2]% vs [56.1±3.4]%,[46.2±4.7]%,P0.05).Conclusion: aAPC can effectively activate CD8+T cells of HCC patients,and the resultant CTLs not only secrete IFN-γ but also efficiently kill human liver cancer BEL7402 cells and self-HCC cells." @default.
- W2354306255 created "2016-06-24" @default.
- W2354306255 creator A5025319461 @default.
- W2354306255 date "2012-01-01" @default.
- W2354306255 modified "2023-09-22" @default.
- W2354306255 title "Artificial antigen presenting cells in vitro activate CD8~+ T cells of hepatocellular carcinoma" @default.
- W2354306255 hasPublicationYear "2012" @default.
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