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- W2354333975 abstract "The gene encoding the viral nucleocapsid(N) was amplified by RT-PCR from IHNV-ZYX strain and cloned into pET30b vector.The expression of recombinant plasmid pET30b-N in E.coli Rosetta(DE3) was induced and detected by SDS-PAGE analysis.The molecular weight of expressed recombinant N protein was approximately 48 KD as predicted.The inclusion body containing fusion protein was extracted and immunized in rabbits to obtain the antisera against N protein.Antigenicity of N protein was analyzed by indirect ELISA and Western-blotting.The results showed that the expressed N protein has immunogenical and antigenical characters as native IHNV N protein.This study has laid an important material foundation for further studying the N gene function of IHNV-ZYX in immune protection,establishing sensitive and efficient methods in detecting infectious hematopoietic necrosis,and manufacturing genetic engineering vaccine." @default.
- W2354333975 created "2016-06-24" @default.
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- W2354333975 date "2011-01-01" @default.
- W2354333975 modified "2023-09-23" @default.
- W2354333975 title "Prokaryotic expression and antigenicity analysis of the nucleocapsid gene of infectious hematopoietic necrosis virus" @default.
- W2354333975 hasPublicationYear "2011" @default.
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