Matches in SemOpenAlex for { <https://semopenalex.org/work/W2354338905> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W2354338905 abstract "Objective To construct a recombinant DNA vaccine plasmid with protective antigen gene of vesicular stomatitis virus(VSV) and determine its immunogenicity. Methods Amplify the gene encoding G protein from the genome of VSV by RT-PCR to construct recombinant plasmid pIRES-G1500. Transfect BHK-21 cells with pIRES-G1500 and identify the expressed product by indirect IFA, PCR, SDS-PAGE, ELISA and Western blot. Immunize BALB/c mice with pIRES-G1500 and determine the cellular and humoral immune responses. Results The constructed recombinant DNA vaccine plasmid pIRES-G1500 was correctly expressed in BHK-21 cells. The plasmid stimulated the significant increases of the numbers of splenic cells and T lymphocyte subclasses as well as the killing activity of CTL, and induced high specific antibody titer against VSV. Conclusion The constructed recombinant DNA plasmid might be used as a candidate vaccine against VSV." @default.
- W2354338905 created "2016-06-24" @default.
- W2354338905 creator A5075046413 @default.
- W2354338905 date "2008-01-01" @default.
- W2354338905 modified "2023-09-24" @default.
- W2354338905 title "Construction and Immunogenictiy of Recombinant DNA Vaccine Plasmid with Protective Antigen Gene of Vesicular Stomatitis Virus" @default.
- W2354338905 hasPublicationYear "2008" @default.
- W2354338905 type Work @default.
- W2354338905 sameAs 2354338905 @default.
- W2354338905 citedByCount "0" @default.
- W2354338905 crossrefType "journal-article" @default.
- W2354338905 hasAuthorship W2354338905A5075046413 @default.
- W2354338905 hasConcept C104317684 @default.
- W2354338905 hasConcept C147483822 @default.
- W2354338905 hasConcept C153911025 @default.
- W2354338905 hasConcept C159047783 @default.
- W2354338905 hasConcept C167508917 @default.
- W2354338905 hasConcept C203014093 @default.
- W2354338905 hasConcept C22744801 @default.
- W2354338905 hasConcept C2522874641 @default.
- W2354338905 hasConcept C2779057330 @default.
- W2354338905 hasConcept C2780868878 @default.
- W2354338905 hasConcept C32611913 @default.
- W2354338905 hasConcept C40767141 @default.
- W2354338905 hasConcept C54355233 @default.
- W2354338905 hasConcept C86803240 @default.
- W2354338905 hasConceptScore W2354338905C104317684 @default.
- W2354338905 hasConceptScore W2354338905C147483822 @default.
- W2354338905 hasConceptScore W2354338905C153911025 @default.
- W2354338905 hasConceptScore W2354338905C159047783 @default.
- W2354338905 hasConceptScore W2354338905C167508917 @default.
- W2354338905 hasConceptScore W2354338905C203014093 @default.
- W2354338905 hasConceptScore W2354338905C22744801 @default.
- W2354338905 hasConceptScore W2354338905C2522874641 @default.
- W2354338905 hasConceptScore W2354338905C2779057330 @default.
- W2354338905 hasConceptScore W2354338905C2780868878 @default.
- W2354338905 hasConceptScore W2354338905C32611913 @default.
- W2354338905 hasConceptScore W2354338905C40767141 @default.
- W2354338905 hasConceptScore W2354338905C54355233 @default.
- W2354338905 hasConceptScore W2354338905C86803240 @default.
- W2354338905 hasLocation W23543389051 @default.
- W2354338905 hasOpenAccess W2354338905 @default.
- W2354338905 hasPrimaryLocation W23543389051 @default.
- W2354338905 hasRelatedWork W1186271811 @default.
- W2354338905 hasRelatedWork W1475002388 @default.
- W2354338905 hasRelatedWork W1968020609 @default.
- W2354338905 hasRelatedWork W1997459605 @default.
- W2354338905 hasRelatedWork W2348985944 @default.
- W2354338905 hasRelatedWork W2349814155 @default.
- W2354338905 hasRelatedWork W2350989161 @default.
- W2354338905 hasRelatedWork W2355396218 @default.
- W2354338905 hasRelatedWork W2357877369 @default.
- W2354338905 hasRelatedWork W2359288867 @default.
- W2354338905 hasRelatedWork W2377805670 @default.
- W2354338905 hasRelatedWork W2381969331 @default.
- W2354338905 hasRelatedWork W2384625109 @default.
- W2354338905 hasRelatedWork W2385495579 @default.
- W2354338905 hasRelatedWork W2391775995 @default.
- W2354338905 hasRelatedWork W2393385594 @default.
- W2354338905 hasRelatedWork W2394247790 @default.
- W2354338905 hasRelatedWork W2394321048 @default.
- W2354338905 hasRelatedWork W250367719 @default.
- W2354338905 hasRelatedWork W3031647227 @default.
- W2354338905 isParatext "false" @default.
- W2354338905 isRetracted "false" @default.
- W2354338905 magId "2354338905" @default.
- W2354338905 workType "article" @default.