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- W2354393482 abstract "Objective To study the effect of Bifidobacterium bifidum(B.bifidum) on cell growth and IL-8 secretion in intestinal epithelial cell line HT29.Methods B.bifidums were anaerobically cultivated.HT29 cells were divided into four groups:normal control group(NC group),low dose B.bifidum co-cultivated group(LB group) and high dose B.bifidum co-cultivated group(HB group) and RV infected control group(RIC group).Inactivated B.bifidum were respectively added into LB group and HB group with final concentration of 1×106 CFU/ml and 1×109 CFU/ml and HT29 cells were infected with RV in RIC group.The state of cell was observed and the number of living cell were evaluated by MTT after 24 h;Cell supernatants were collected 6 h,24 h post cocultivation,and examined for secretion level of IL-8 by ELISA.Results There were no change in LB group and HB group 24 h post cocultivation,but the RIC group showed CPE at 24 h and the number of living cell decreased significantly compared with NC group,LB group and HB group.The other three groups had a higher IL-8 level(P0.05) than NC group 6 and 24 h post cocultivation(P0.05),in which,HB group increased significantly compared with LB group.But markedly lower level of both two than RIC group(P0.05).Conclusions B.bifidum could induce the secretion of cytokines IL-8 in HT29 cells with no damage of cell,but the change significantly little than which the pathogenic microorganism induced.It displays the different effect of B.bifidum and pathogenic microorganism on IECs,the promotion of IL-8 secretion may involve in the development and maturation of mucosal immunity." @default.
- W2354393482 created "2016-06-24" @default.
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- W2354393482 date "2008-01-01" @default.
- W2354393482 modified "2023-09-22" @default.
- W2354393482 title "Effect of Bifidobacterium on cell growth and IL-8 secretion in IECs" @default.
- W2354393482 hasPublicationYear "2008" @default.
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