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- W2354403971 abstract "OBJECTIVE To develop a simple and reproducible high-performance liquid chromatography (HPLC) method for determination of cyclosporine A (CsA, also known as cyclosporin A) in aqueous humor of rabbit eyes using CsD as internal standard. METHODS The pretreatment involved one step of liquid- liquid extraction with ether from aqueous humor samples. Chromatography was carried out using an ODS column which was controlled at 75℃ under isocratic elution with the mobile phase of acetonitrile-deionized water (89:11, v/v)at the flow rate of 0.1mL·min~(-1) and the detector set at 200 nm. RESULTS The mean absolute recovery of CsA from aqueous humor was 86% and the mean relative recovery was 100.7%.The linearity was assessed in the range of 25～500 ng·mL~(-1), with a correlation coefficient of 0.9986. The limit of quantification and detection of the present method were 25 and 10 ng·mL~(-1), respectively. CONCLUSION This method has been used to analyze large numbers of aqueous humor samples of rabbit eyes for ophthalmic research and proved to be rapid with good accuracy and precision." @default.
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- W2354403971 date "2005-01-01" @default.
- W2354403971 modified "2023-09-25" @default.
- W2354403971 title "Measurement of cyclosporine A in aqueous humor of rabbit eyes by HPLC" @default.
- W2354403971 hasPublicationYear "2005" @default.
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