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- W2354425280 abstract "Objective To establish an HPLC method for simultaneous determination of narirutin,naringin and neohesperidin in Citrus Changshan-huyou Y.B.Chang.Methods The chromatographic method was carried out on a Diamnosil C18 column(200 mm×4.6 mm,5 μm)with a mobile phase of methanol-water-glacial acetic acid(V∶V∶V=37∶63∶0.2).The flow rate was 1.0 mL·min-1 and the column temperature was room temperature.The detection wavelength was set at 283 nm.Results The calibration curve was linear within the range of 72-576 mg·L-1(r=0.999 6)for narirutin,113-904 mg·L-1(r=0.999 9)for naringin,and 53-424 mg·L-1(r=0.999 9)for neohesperidin,respectively.The average recoveries of narirutin,naringin and neohesperidin were 98.3%(RSD=1.53%,n=6),98.0%(RSD=1.90%,n=6),and 98.4%(RSD=1.35%,n=6),respectively.The average contents of narirutin,naringin and neohesperidin in Citrus Changshan-huyou Y.B.Chang were 157,289 and 172 mg·L-1,respectively.Conclusions The method is accurate and reproducible,and can be used for the quality control of Citrus Changshan-huyou Y.B.Chang." @default.
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- W2354425280 date "2010-01-01" @default.
- W2354425280 modified "2023-09-27" @default.
- W2354425280 title "Simultaneous determination of contents of narirutin,naringin and neohesperidin in Citrus Changshan-huyou Y.B.Chang by HPLC" @default.
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