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- W2354429553 abstract "Objective To explore the direct effect of 400 mL·L-1 oxygen(O2) on proliferation of human embryo lung fibroblasts(HELFs) cultured in vitro. Methods After HELFs were passaged according to the ratio of 1 to 3 and cultured 24 hours in DMEM with no fetal bovine serum,then randomly divided into air group and 400 mL·L-1 O2 group.Cells were cultured for 5 days.Cells were collected everyday and cellular growth curve was drew;cell division was determined by Giemsa staining after cultured 1 day,3 days and 5 days;the changes of cell cycle were analyzed by flow cytometry after cultured 5 days. Results The concentrations of 400 mL·L-1 O2 promoted cell growth.As compared with air group,the division index was 2.47% and 2.03% in 400 mL·L-1 O2 group,1.90% and 1.65% in air group after treated cells 3 d and 5 d(P0.01,0.05).As compared with air group,the fraction of cells in G0/G1 phase decreased(P0.01) and that in S phase increased(P0.01) in 400 mL·L-1 O2 group,the fraction of cells in G2/M phase had no significant difference(P0.05). Conclusion The concentration of 400 mL·L-1 promote HELFs growth and proliferation." @default.
- W2354429553 created "2016-06-24" @default.
- W2354429553 creator A5040144876 @default.
- W2354429553 date "2012-01-01" @default.
- W2354429553 modified "2023-09-26" @default.
- W2354429553 title "Effects of 400 mL·L~(-1) Oxygen on Proliferation of Human Embryo Lung Fibroblasts Cultured in Vitro" @default.
- W2354429553 hasPublicationYear "2012" @default.
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