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- W2354448909 abstract "In the present paper,the viabillity of 122 strains from 43 species and 24 genera of basidiomycetes cultures subjected to ultra-low temperature freezing and thawing,the effect of cooling rate during freezing,the efficacy of.10% glycerol and 5% DMSO protection in freeze process and the ability for fruit body formation of the selected 41 strains from 23 species after storage in liquid nitrogen for 12—31 months are reported. These cultures are all viable when they are thawed after frozen state and cultivation. Slow freezing controlled at cooling rate at 1℃/min.,gives higher survival rate than rapid freezing which put the samples into liquid nitrogen refrigerator directly and immediately.For example,the average survival rate of cultures protected with glycerol or DMSO using rapid freezing is 81% or 97% respectively,but slow freezing is 94% or 100%. Excepting two Trernella fuciformis strains,the cultures of Agaricus pratensis,Auricularia auricula,Collybia velutipes,Ganoderma capense,G.japonicum,G.lucidum,Hericum erinaceus, Lactarius camphoratus,Lentinus edodes,Pholiota adiposa,P.nameko,Pleurotus cornucopiae,P. cystidiosas,P.florida,P.ostreatus,P.sapidus,P.sojor-caju,Polyporus versicolor,Poria cocos, Volvaria bombycina,V.volvacea and Schizophyllum commune after storage in liquid nitrogen for different time were ratained the ability to form fruit body when they were thawed and cul- tivated in laboratory." @default.
- W2354448909 created "2016-06-24" @default.
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- W2354448909 date "1987-01-01" @default.
- W2354448909 modified "2023-09-26" @default.
- W2354448909 title "THE PRESERVATION OF BASIDIOMYCETES CULTURES BY ULTRA-LOW TEMPERATURE FREEZING" @default.
- W2354448909 hasPublicationYear "1987" @default.
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