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- W2354460213 abstract "The major structure protein of infectious bursal disease virus, VP2, is the major protective of the virus and the point mutations in the variant area located in VP2 lead to the antigen shift and the sepuenced circumvent of vaccination. In this study a VP2 gene was inserted into a Bombyx mori baculovirus transfer vector pBacPAK8 and cotransfected with linear virus Bm BacPAK6 into BmN cells. The BmN cells and the silkworm of fifth instar were infected by the recombinant virus. DNA extracted from the infected cells and the immunogenesis of the infected larvae's blood were examined with dot hybridization and ELISA?Western blotting, respectively. The recombinant VP2 in blood achieved the peak at 5 day post inucolation and the expression amount of the protein in the larvae was obviously higher than that in BmN cells." @default.
- W2354460213 created "2016-06-24" @default.
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- W2354460213 date "2002-01-01" @default.
- W2354460213 modified "2023-09-23" @default.
- W2354460213 title "Expression of VP2 of IBDV with Bombyx mori Baculovirus Expression Vector System" @default.
- W2354460213 hasPublicationYear "2002" @default.
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