Matches in SemOpenAlex for { <https://semopenalex.org/work/W2354495805> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W2354495805 endingPage "649" @default.
- W2354495805 startingPage "646" @default.
- W2354495805 abstract "Objective To investigate the anti-metastatic effect of vascular endothelial growth factor receptor 2 extracellular domain gene-modified dendritic cell （DC-sVEGFR-2） vaccination. Methods Dendritic cells （DC） were electroporated with pcDNA3. 1/sVEGFR-2 plasmid DNA. Expression of sVEGFR-2 was determined by ELISA. For immunization, C57BL/6 mice were intravenously injected three times with 1 × 10^5 cells per mouse of DC, pcDNA3.1-transfected DC （ DC-vector）, DC-sVEGFR-2, or 100 μl of PBS at 7- day intervals. At 10 days after the last immunization, the immunized mice were subjected to assessment of cytotoxic T lymphocyte （CTL） response to VEGFR-2, alginate bead analysis of tumor cell-induced angiogenesis, and observation of the antl-metastatic effect in B16 melanoma metastasis model. CTL activity was determined by a standard 4-h ^51Cr release assay against VEGFR-2^＋ vascular endothelial cell line H5V, 3LL cells stably transfected with pcDNA3.1/sVEGFR-2 （3LL-sVEGFR-2）, and VEGFR-2- cell lines EL-4 and 3LL. Monoclonal antibodies GK1.5 anti-CD4 and 2.43 anti-CD8 were used to deplete in vivo CD4^＋ T cells and CD8^＋ T cells, respectively. Results DC-sVEGFR-2 could effectively express sVEGFR-2, whereas DC-vector and DC could not. Immunization of mice with DC-sVEGFR-2 significantly induce CTL activity against VEGFR-2 ^＋ cell lines H5V and 3LL-sVEGFR-2, however, no significant CTL activity was observed when VEGFR-2- syngeneic cell lines EL-4 and 3LL were used as target cells, implying this CTL activity was VEGFR-2 specific. Alginate bead analysis of in vivo neoangiogenesis showed that the inhibition reached 50% in mice vaccinated with DC-sVEGFR-2 compared with mice vaccinated with DC, DC-vector or PBS. Anti-metastatic experiment showed that profound reduction in pulmonary metastases was found in mice immunized with DC-sVEGFR-2, while mice immunized with PBS, DC, DC-vector developed extensive pulmonary metastases. The number of tumor nodules on lung surface decreased by 81.9% in mice immunized with DC-sVEGFR-2 when compared with mice immunized with DC-vector （49.7± 12.7 vs. 9.0±3.2）. In vivo T cell subset depletion experiments showed that the anti-metastatic effect of DC-sVEGFR-2 vaccination was abrogated in CD8^＋ T cell-depleted but not in CD4^＋ T cell-depleted mice. Conclusion Immunization of mice with DC-sVEGFR-2 could break self-tolerance and induce a significant CTL response to VEGFR-2, leading to profound inhibition of tumor-cell induced angiogenesis and metastasis. This anti-metastatic effect is mainly mediated by CD8^＋ T cells.Key words: Vascular endothelial growth factor receptor 2; Dendritic cell; Angiogenesis; Melanoma" @default.
- W2354495805 created "2016-06-24" @default.
- W2354495805 creator A5002032950 @default.
- W2354495805 creator A5019387317 @default.
- W2354495805 creator A5027767246 @default.
- W2354495805 date "2006-09-01" @default.
- W2354495805 modified "2023-09-27" @default.
- W2354495805 title "[Anti-metastatic effect of vascular endothelial growth factor receptor 2 extracellular domain gene-modified dendritic cell vaccination in murine model with experimental pulmonary metastasis]." @default.
- W2354495805 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17274366" @default.
- W2354495805 hasPublicationYear "2006" @default.
- W2354495805 type Work @default.
- W2354495805 sameAs 2354495805 @default.
- W2354495805 citedByCount "0" @default.
- W2354495805 crossrefType "journal-article" @default.
- W2354495805 hasAuthorship W2354495805A5002032950 @default.
- W2354495805 hasAuthorship W2354495805A5019387317 @default.
- W2354495805 hasAuthorship W2354495805A5027767246 @default.
- W2354495805 hasConcept C147969180 @default.
- W2354495805 hasConcept C153911025 @default.
- W2354495805 hasConcept C154317977 @default.
- W2354495805 hasConcept C167672396 @default.
- W2354495805 hasConcept C202751555 @default.
- W2354495805 hasConcept C203014093 @default.
- W2354495805 hasConcept C2777701055 @default.
- W2354495805 hasConcept C2778170410 @default.
- W2354495805 hasConcept C2780394083 @default.
- W2354495805 hasConcept C502942594 @default.
- W2354495805 hasConcept C54009773 @default.
- W2354495805 hasConcept C54355233 @default.
- W2354495805 hasConcept C55493867 @default.
- W2354495805 hasConcept C71924100 @default.
- W2354495805 hasConcept C81885089 @default.
- W2354495805 hasConcept C86803240 @default.
- W2354495805 hasConcept C8891405 @default.
- W2354495805 hasConceptScore W2354495805C147969180 @default.
- W2354495805 hasConceptScore W2354495805C153911025 @default.
- W2354495805 hasConceptScore W2354495805C154317977 @default.
- W2354495805 hasConceptScore W2354495805C167672396 @default.
- W2354495805 hasConceptScore W2354495805C202751555 @default.
- W2354495805 hasConceptScore W2354495805C203014093 @default.
- W2354495805 hasConceptScore W2354495805C2777701055 @default.
- W2354495805 hasConceptScore W2354495805C2778170410 @default.
- W2354495805 hasConceptScore W2354495805C2780394083 @default.
- W2354495805 hasConceptScore W2354495805C502942594 @default.
- W2354495805 hasConceptScore W2354495805C54009773 @default.
- W2354495805 hasConceptScore W2354495805C54355233 @default.
- W2354495805 hasConceptScore W2354495805C55493867 @default.
- W2354495805 hasConceptScore W2354495805C71924100 @default.
- W2354495805 hasConceptScore W2354495805C81885089 @default.
- W2354495805 hasConceptScore W2354495805C86803240 @default.
- W2354495805 hasConceptScore W2354495805C8891405 @default.
- W2354495805 hasIssue "9" @default.
- W2354495805 hasLocation W23544958051 @default.
- W2354495805 hasLocation W23544958052 @default.
- W2354495805 hasOpenAccess W2354495805 @default.
- W2354495805 hasPrimaryLocation W23544958051 @default.
- W2354495805 hasRelatedWork W1990095452 @default.
- W2354495805 hasRelatedWork W1997080388 @default.
- W2354495805 hasRelatedWork W2026763954 @default.
- W2354495805 hasRelatedWork W2086090066 @default.
- W2354495805 hasRelatedWork W2101728703 @default.
- W2354495805 hasRelatedWork W2325832391 @default.
- W2354495805 hasRelatedWork W2377205104 @default.
- W2354495805 hasRelatedWork W2379749676 @default.
- W2354495805 hasRelatedWork W2383253575 @default.
- W2354495805 hasRelatedWork W2384399723 @default.
- W2354495805 hasRelatedWork W2388802421 @default.
- W2354495805 hasRelatedWork W2391915694 @default.
- W2354495805 hasRelatedWork W2396595811 @default.
- W2354495805 hasRelatedWork W2404726169 @default.
- W2354495805 hasRelatedWork W2411310721 @default.
- W2354495805 hasRelatedWork W2413983463 @default.
- W2354495805 hasRelatedWork W2765318938 @default.
- W2354495805 hasRelatedWork W3144055737 @default.
- W2354495805 hasRelatedWork W3152070394 @default.
- W2354495805 hasRelatedWork W3179233595 @default.
- W2354495805 hasVolume "28" @default.
- W2354495805 isParatext "false" @default.
- W2354495805 isRetracted "false" @default.
- W2354495805 magId "2354495805" @default.
- W2354495805 workType "article" @default.