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- W2354503429 abstract "Objective:To investigate the antibiotic resistance and β-Lactamase genotype of Escherichia coli(E.coli) from community-acquired urinary tract infections.Methods:A total of 39 E.coli isolates from community-acquired urinary tract infections were collected.The antimicrobial susceptibility was tested in vitro.Six β-Lactamase genes(CTX-M,TEM,SHV,DHA,EBC and CIT) screened by PCR,and gene subtype was confirmed by direct sequencing.Results:Of 39 E.coli isolates,21(53.8%) were ESBL producer.All ESBL-producing isolates were resistant to common β-lactam antibiotics and more than 70% were resistant to Gentamicin,Ciprofloxacin and Levofloxacin,Which was two-times as compared to ESBL non-producer.No isolates were found resistant to Piperacillin-tazobactam,Cefotetan,Imipenem and Amikacin.The β-Lactamase genes screening test showed that the TEM prevalence was highest than other genes(25/39,64.1%),followed by CTX-M(11/39,28.2%).SHV(1/39,2.6%),CIT(4/39,10.3%),EBC(2/39,5.1%) and DHA(3/39,7.7%) prevalence is lower than CTX-M and TEM.Nevertheless,CTX-M were only found in ESBL producers(11/21,52.4%),but not in ESBL non-producer.However,TEM prevalence in ESBL-positive isolates was similar to ESBL-negative ones [13/21(61.9%) versus 12/18(66.7%),P0.05],and these isolates all harbored TEM-1 subtype.Genotype analysis showed that TEM(4/21,19%),CTX-M(3/21,14.3%) and TEM+CTX-M(4/21,19%) were the main types in ESBL producers,and single TEM was the main type in ESBL non-producers(11/18,61.1%).Conclusion:The ESBL-producing isolates account for more than half of E.coli from community-acquired urinary tract infections,which need to be given more attention.TEM and CTX-M are the main β-Lactamase gene type in these E.coli isolates." @default.
- W2354503429 created "2016-06-24" @default.
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- W2354503429 date "2010-01-01" @default.
- W2354503429 modified "2023-09-28" @default.
- W2354503429 title "Antibiotic resistance and β-Lactamase genotype of Escherichia coli from community-acquired urinary tract infections" @default.
- W2354503429 hasPublicationYear "2010" @default.
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