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- W2354569081 abstract "Nostoc flagelliforme is a kind of terrestrial cyanobacterium of great economic value.C-phycocyanin is a kind of blue protein belonging to the photosynthetic apparatus,and of fluorescence molecular probe both with the characteristics of absorption spectra and fluorescence spectra.In the study,with N.flagelliforme as materials,the effects of the types of extraction liquid and concentration of ammonium sulfate on the extraction of C-phycocyanin were compared.An efficient procedure for isolation and purification of C-phycocyanin was studied and some of its properties were also investigated.The results showed that 50 mmol/L K phosphate buffer(pH 7.2) was more ideal extraction liquid,with solid ammonium sulfate at 40%～50% saturation was more ideal concentration for precipitation,and then followed by ion exchange chromatography on DEAE-Toyopeal 650 S column and gel filtration chromatography on SuperdexTM 200 column,pure C-phycocyanin was finally obtained from N.flagelliforme with purity ratio(A615/A280) 6.2.The max absorption and fluorescence emission of purified C-phycocyanin was at 615 nm and at 649 nm respectively.C-phycocyanin was composed of α and β subunits,and the molecular mass of α and β subunits were 18 051.17 and 19 142.27 Da respectively.Therefore,the more ideal procedure of extracting C-phycocyanin from N.flagelliforme was as follows:algae power →soaking with 50 mmol/L K phosphate buffer(pH 7.2) →crushing cells by French pressure(1 500 kg/cm2) →salting out with 40%～50% saturated ammonium sulfate→DEAE-Toyopeal 650 S column chromatography→SuperdexTM 200 column chromatography →gaining C-phycocyanin with high purity." @default.
- W2354569081 created "2016-06-24" @default.
- W2354569081 creator A5083374724 @default.
- W2354569081 date "2008-01-01" @default.
- W2354569081 modified "2023-09-26" @default.
- W2354569081 title "Isolation and Purification of C-phycocyanin from Nostoc flagelliforme" @default.
- W2354569081 hasPublicationYear "2008" @default.
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