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- W2354574613 abstract "Objective To investigate the phenomena of autophagy in brachytherapy of 125I seeds induced cytotoxicity in human U251 glioma cells. Methods Autophagy of U251 glioma cells was demonstrated by enhanced fluorescence intensity of monodansylcadaverine( MDC),enhanced fluorescence intensity of lysosome with Lysosome tracker Red,as well as elevated expression of autophagy-related protein lingt chain 3( LC3-Ⅱ). Results Brachytherapy with 125I seeds decreased the viability of U251 cells in a dose-and time-dependent manner. Autophagy was induced in U251 cells after brachytherapy with 125I seeds,which was evidenced by enhanced fluorescence intensity of MDC and activity of lysosome. Western blot analysis indicated that brachytherapy with low dose rate 125I seeds also increased protein levels of microtubule-associated protein light chain3(LC3-Ⅱ). Conclusion Autophagy was induced in U251 cell During the progress of brachytherapy with 125I seeds." @default.
- W2354574613 created "2016-06-24" @default.
- W2354574613 creator A5032841958 @default.
- W2354574613 date "2013-01-01" @default.
- W2354574613 modified "2023-09-24" @default.
- W2354574613 title "Phenomena of autophagy in human U251 glioma cells after brachytherapy with ~(125)I seeds" @default.
- W2354574613 hasPublicationYear "2013" @default.
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