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- W2354575700 abstract "VTS76, a eukaryotic expression recombinant plasmid of an antigenetic gene of cys ticercus cellulosae was constructed. VTS76 was transformed into E.coli DH5α competent cells. The recombinant plasmid were extracted and digested with Hin dⅢ and PstⅠ to identified the correct orientation. VTS76 plasmid DNA was entrapped with LipofectamineTM Reagen and expressed in Cos7 cells in vit ro. The expressed products of VTS76 in Cos7 cells were analyzed by ELISA with ra bbit antisera against cysticercus in 24h and 72h. mRNA of the transfected C os7 cells was extracted and detected by northern blotting. Results show that VTS 76 could express full-length antigen in vitro in Cos7 cells. A transcript on th e northern blot that comes only from the recombinant plasmid containing TS76 gen e in Cos 7 cells. So the plasmid VTS76 encoding immunogenic protein gene of cysticercus c ellulosae could be employed as a candidate for DNA vaccination against taenia so lium crysticerosis." @default.
- W2354575700 created "2016-06-24" @default.
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- W2354575700 date "2001-01-01" @default.
- W2354575700 modified "2023-09-27" @default.
- W2354575700 title "THE EUKARYOTIC EXPRESSION OF TS76 ANTIGENETIC GENE OF CYSTICERCUS CELLULOSAE" @default.
- W2354575700 hasPublicationYear "2001" @default.
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