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- W2354607002 abstract "Objective To establish the comet assay in vivo method used for rat liver and peripheral blood lymphocyte cells and detect the genotoxicity of ethyl methanesulfonate(EMS) and cyclophosphamide(CPA) Methods Sprague-Dawley(SD) male rats were treated with series doses of EMS and CPA.Peripheral blood sample and tissue in left lobe of liver were collected to prepare single cell gel suspension and pave the slides.The slides were processed with complex steps containing lysis,unwinding,electrophoresis,neutralizing,and dehydrating to get the scoring specimen.To dye the specimens,observe the comet cells under fluorescence microscope(40×),and then photograph.The cell images were analyzed with special software and determined by electrophoresis.Results EMS showed positive reaction while CPA was negative in two tissues.It was convinced that EMS was appropriate as positive reagent in this assay.Conclusion The method of rat comet assay in vivo is successfully established and it could be used to genotoxicity detection." @default.
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- W2354607002 date "2012-01-01" @default.
- W2354607002 modified "2023-09-23" @default.
- W2354607002 title "Establishment and application of comet assay in vivo method in rats" @default.
- W2354607002 hasPublicationYear "2012" @default.
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