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- W2354713922 abstract "With Zizyphus jujuba as transformed materials ,transgenic jujube is obtaine d through the anti-ACC gene transformation by Agrobacterium tumefactions .Th e younger stems precultured for 1 d are infected with Agrobacterium tumefactio ns and then the stems were used to introduce shoots on the medium supplemented with 0.15 mg/L NAA,1.75 mg/L ZT,10 mg/L Km,500 mg/L Carb.The Kanamyci-resistan t shoots are further selected on the mediums containing 15 mg/L Km,on which the shoots grow,reproduce,root and whole plants are obtained.With the PCR test ,se ven positive plants are selected from those Kanamycin-resistant plants,and the southern hybridization shows that the foreign gene has been integrated into th e six of seven positive plants'genome,and Real time PCR reveals that anti-ACC g ene is expressed in the five of six plants." @default.
- W2354713922 created "2016-06-24" @default.
- W2354713922 creator A5050865543 @default.
- W2354713922 date "2004-01-01" @default.
- W2354713922 modified "2023-09-25" @default.
- W2354713922 title "Transformation of Zizyphus jujuba Hetau with Antisense ACC Synthase Gene" @default.
- W2354713922 hasPublicationYear "2004" @default.
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