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- W2354811529 abstract "To exploit a genetic engineered antiviral medicine against the porcine immune suppression diseases, mature porcine interferon-alpha (PoIFN-α) gene was amplified by PCR. The PCR product, which comprised 501 bp nucleotides encoding 166 amino acids, was sequenced and cloned into the prokaryotic expression vector pQE30. The expressed recombinant PoIFN-α (rPoIFN-α) protein was purified under denatured condition and refolded in a novel re-naturation buffer. The CPE suppression by rPoIFN-α was tested for vesicular stomatitis virus (VSV) in porcine kidney 15 (PK15) cell line and the high-pathogenic porcine reproductive and respiratory syndrome virus (PRRSV) in Marc-145 cell line. The antiviral activity of rPoIFN-α on PK15 cell line was 1.4482×104 IU/mL, and the lowest effective dose of rPoIFN-α to inhibit the proliferation of high-pathogenic PRRSV was 640 U/mL (2.3×104 U/mg). These results indicated that interferon-alpha could be used as an antiviral agent to interfere the high-pathogenic PRRSV and other porcine virus infections." @default.
- W2354811529 created "2016-06-24" @default.
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- W2354811529 date "2008-01-01" @default.
- W2354811529 modified "2023-09-24" @default.
- W2354811529 title "Antiviral activity of the porcine interferon-alpha protein against high-pathogenic porcine reproductive and respiratory syndrome virus" @default.
- W2354811529 hasPublicationYear "2008" @default.
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