Matches in SemOpenAlex for { <https://semopenalex.org/work/W2354840373> ?p ?o ?g. }
Showing items 1 to 49 of
49
with 100 items per page.
- W2354840373 abstract "To fuse the gene encoding the surface antigen P1 with hspB of Coxiella burnetii and to express the p1 hspB fusion gene in E. coli cells. The p1 and hspB fragment was amplified from C. burnetii genomic DNA by PCR and the gene fragments were ligated into the prokaryotic expression vector pQE30 to construct pQE30/p1 and pQE30/hspB, respectively. The p1 from pQE30/p1 was fused with hspB of pQE30/hspB to construct pQE30/p1 hspB. The expression of p1 hspB fusion gene of pQE30/p1 hspB was induced with IPTG in E. coli cells.The P1 HspB fusion protein was observed to be about 83 kDa in SDS PAGE and 12.1% of total cell protein in scanning analysis; the fusion protein was recognized by the serum from mice infected with C. burnetii in immunoblot assay.The p1 hspB fusion gene of pQE30/p1 hspB is expressed in E. coli cells by inducing of IPTG and the resulting P1 HspB fusion protein has ability to react to the antibodies to C. burnetii; the P1 HspB may be a new candidate of subunit vaccines against Q fever." @default.
- W2354840373 created "2016-06-24" @default.
- W2354840373 creator A5036994812 @default.
- W2354840373 date "2004-01-01" @default.
- W2354840373 modified "2023-09-23" @default.
- W2354840373 title "Expression of surface antigen gene p1 fused with hspB of Coxiella burnetii in E.coli" @default.
- W2354840373 hasPublicationYear "2004" @default.
- W2354840373 type Work @default.
- W2354840373 sameAs 2354840373 @default.
- W2354840373 citedByCount "0" @default.
- W2354840373 crossrefType "journal-article" @default.
- W2354840373 hasAuthorship W2354840373A5036994812 @default.
- W2354840373 hasConcept C104317684 @default.
- W2354840373 hasConcept C123894998 @default.
- W2354840373 hasConcept C153911025 @default.
- W2354840373 hasConcept C40767141 @default.
- W2354840373 hasConcept C54355233 @default.
- W2354840373 hasConcept C86803240 @default.
- W2354840373 hasConceptScore W2354840373C104317684 @default.
- W2354840373 hasConceptScore W2354840373C123894998 @default.
- W2354840373 hasConceptScore W2354840373C153911025 @default.
- W2354840373 hasConceptScore W2354840373C40767141 @default.
- W2354840373 hasConceptScore W2354840373C54355233 @default.
- W2354840373 hasConceptScore W2354840373C86803240 @default.
- W2354840373 hasOpenAccess W2354840373 @default.
- W2354840373 hasRelatedWork W2038465288 @default.
- W2354840373 hasRelatedWork W2130861239 @default.
- W2354840373 hasRelatedWork W2253063658 @default.
- W2354840373 hasRelatedWork W2276385301 @default.
- W2354840373 hasRelatedWork W2354936228 @default.
- W2354840373 hasRelatedWork W2367342778 @default.
- W2354840373 hasRelatedWork W2389385091 @default.
- W2354840373 hasRelatedWork W2402744584 @default.
- W2354840373 hasRelatedWork W2408800467 @default.
- W2354840373 hasRelatedWork W2409611724 @default.
- W2354840373 hasRelatedWork W2409997549 @default.
- W2354840373 hasRelatedWork W2410714667 @default.
- W2354840373 hasRelatedWork W2411044105 @default.
- W2354840373 hasRelatedWork W2427939647 @default.
- W2354840373 hasRelatedWork W2428363270 @default.
- W2354840373 hasRelatedWork W2901761664 @default.
- W2354840373 hasRelatedWork W2978702265 @default.
- W2354840373 hasRelatedWork W2994483582 @default.
- W2354840373 hasRelatedWork W3145009602 @default.
- W2354840373 hasRelatedWork W2187056604 @default.
- W2354840373 isParatext "false" @default.
- W2354840373 isRetracted "false" @default.
- W2354840373 magId "2354840373" @default.
- W2354840373 workType "article" @default.