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- W2354864354 abstract "Objective: To construct a plasmid containing an enhanced green fluorescence protein(EGFP) reporter gene as the effective vector for preparing murine granulocyte macrophage colony stimulating factor(mGM-CSF) secreting tumor vaccines.Methods: A pIRES2-EGFP-mGM plasmid,harboring a mGM-CSF gene and a EGFP gene,was designed and constructed by routine molecular cloning techniques.DNA sequence analysis and restrict digest were performed to confirm that EGFP tagged mGM-CSF gene was correctly inserted into pIRES2 vector,pIRES2-EGFP-mGM plasmid was further transferred into LA795 cells,and the expression of EGFP and mGM-CSF genes was examined by way of fluorescent microscopy and ELISA respectively.Results: Restriction analysis showed that the structure of pIRES2-EGFP-mGM plasmid was exactly the same as anticipated.Further result indicated that both EGFP and mGM-CSF genes were simultaneously expressed in LA795 cells.Conclusion: A plasmid is established as the vector for studying the mGM-CSF-secreting tumor vaccines,in which EGFP gene can serve as a reporter gene reflecting the expression of mGM-CSF gene." @default.
- W2354864354 created "2016-06-24" @default.
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- W2354864354 date "2007-01-01" @default.
- W2354864354 modified "2023-09-25" @default.
- W2354864354 title "Construction of pIRES2-EGFP-mGM plasmid and its application in preparing GM-CSF-secreting tumor vaccine" @default.
- W2354864354 hasPublicationYear "2007" @default.
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